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BD 560905 Alexa Fluor® 647 Mouse anti-Human CD127 Clone HIL-7R-M21(RUO)

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  • 商品货号 560905
  • 商品库存 10000
  • 上架时间: 2017-03-09
  • 商品重量 1克
  • 此商品可使用:1200积分
  • 数量:
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 Brand

BD Pharmingen™
Alternative Name
IL-7R; IL7R; IL7RA; IL-7Rα; IL-7R-alpha; Interleukin-7 Receptor alpha
Vol. Per Test
20 µl
Isotype
Mouse IgG1, κ
Reactivity
Human (QC Testing) 
Application
Flow cytometry (Routinely Tested) 
Immunogen
Human IL-7R Recombinant Protein
Storage Buffer
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
Regulatory Status

RUO

 

 

 

 

 

 

 

 

 

 

 

DESCRIPTION

 

The hIL-7R-M21 monoclonal antibody specifically binds to the 60-90 kDa glycoprotein, CD127. CD127 is also known as the IL-7 receptor alpha (IL-7Rα) subunit. The IL-7 receptor complex is a heterodimer composed of CD127 and the common gamma chain (γc, CD132), shared by other cytokine receptors (IL-2R, IL-4R, IL-9R, IL-15R, and IL-21R). CD127 is expressed on thymocytes, T- and B-cell progenitors, mature T cells, and some lymphoid and myeloid cells. In vitro experiments show the expression of CD127 is down-regulated following T cell activation. Studies indicate that the IL-7 Receptor plays an important role in the proliferation and differentiation of mature T cells. Recently, it has been shown that low surface expression of CD127, in combination with intermediate to high surface expression of CD25, the α chain of the IL-2 receptor complex, can distinguish between human regulatory and conventional CD4+ T cells in human adult and cord blood, lymph nodes and thymus.

 

 

FORMAT

Format
Alexa Fluor® 647
Excitation Source
Red 633 nm
Excitation Max
650 nm
Emission Max
668 nm

Alexa Fluor® 647 conjugates are highly photostable and remain fluorescent over a broad pH range. The excitation and emission maxima are nearly identical to those of APC. However, APC tends to be brighter while Alexa Fluor® 647 is more optimal for intracellular applications. This fluorochrome exhibits uncommon photostability, making it an ideal choice for use in fluorescence microscopy. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously.


PREPARATION AND STORAGE

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

PRODUCT NOTICES

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  9. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
 
 
  • BD 560905 Alexa Fluor® 647 Mouse anti-Human CD127 Clone HIL-7R-M21(RUO)
    • 产品名称:BD 560905 Alexa Fluor® 647 Mouse anti-Human CD127 Clone HIL-7R-M21(RUO)
    • 产品品牌:BD 560905 Alexa Fluor® 647 Mouse anti-Human CD127 Clone HIL-7R-M21(RUO)
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BD 560905 Alexa Fluor® 647 Mouse anti-Human CD127 Clone HIL-7R-M21(RUO)
BD 560905 Alexa Fluor® 647 Mouse anti-Human CD127 Clone HIL-7R-M21(RUO)

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